KLRG1 Recombinant antibody

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  WB analysis using 84785-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84785-4-RR (KLRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84785-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84785-4-RR (KLRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

KLRG1 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84785-4-RR

Clone No.242327B12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

MAFA, Killer cell lectin-like receptor subfamily G member 1, ITIM-containing receptor MAFA-L, C-type lectin domain family 15 member A, CLEC15A

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse splenocytes, Raji cells, NK-92 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84785-4-RR targets KLRG1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: killer cell lectin-like receptor subfamily G, member 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 30-38 kDa
• GenBank Accession Number: NM_005810.4
• Gene Symbol: KLRG1
• Gene ID (NCBI): 10219
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96E93-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLRG1 (killer cell lectin-like receptor subfamily G, member 1) is a C-type lectin inhibitory receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. KLRG1 is expressed by subsets of NK and T cells, existing both as a monomer and as a disulfide-linked homodimer (PMID: 10741410; 19604491). It is considered to be a cell differentiation marker for NK and T cells and is strongly induced by viral and other infections (PMID: 20373518). Through interactions with members of the cadherin family, KLRG1 plays an inhibitory role in NK- and T-cell function (PMID: 19009530; 20373518). Because of the glycosylation and disulfide bonds of the KLRG1 proteins, the observed molecular weights are typically 30-38 kDa (PMID: 17404256).