Anti-Rat KLRB1/CD161 Rabbit Recombinant Antibody

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  FC experiment of rat splenocytes using 98249-1-RR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat KLRB1/CD161 Rabbit Recombinant Antibody (98249-1-RR, Clone: 241840F3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of rat splenocytes using 98249-1-RR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat KLRB1/CD161 Rabbit Recombinant Antibody (98249-1-RR, Clone:241840F3)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98249-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98249-1-RR against Rat KLRB1/CD161 were performed. The affinity constant is 88.8 pM.

KLRB1/CD161 {ptg:Brand} Recombinant Antibody for FC

Cat No. 98249-1-RR

Clone No.241840F3

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC

Synonyms

Klrb1a, Killer cell lectin-like receptor subfamily B member 1A, CD161a, CD161 antigen-like family member A, Antigen 3.2.3

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Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98249-1-RR targets KLRB1/CD161 in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: killer cell lectin-like receptor subfamily B, member 1A
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: NM_001010964.1
• Gene Symbol: Klrb1a
• Gene ID (NCBI): 362443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P27471
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).