KLK3/PSA Polyclonal antibody, PBS Only

KLK3/PSA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10679-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 10679-1-AP (same clone as 10679-1-PBS)

  DU 145(PSA-), PC-3(PSA-) and LNCaP (PSA+) cell lysates were subjected to SDS PAGE followed by western blot with 10679-1-AP (KLK3/PSA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

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  WB analysis of Transfected HEK-293 using 10679-1-AP (same clone as 10679-1-PBS)

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10679-1-AP (KLK3/PSA antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

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  IP experiment of LNCaP using 10679-1-AP (same clone as 10679-1-PBS)

  IP result of anti-KLK3/PSA (IP:10679-1-AP, 4ug; Detection:10679-1-AP 1:10000) with LNCaP cells lysate 1520 ug. This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10679-1-AP (same clone as 10679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10679-1-AP (same clone as 10679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10679-1-AP (same clone as 10679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10679-1-AP (same clone as 10679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10679-1-AP (same clone as 10679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10679-1-AP (same clone as 10679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10679-1-AP (same clone as 10679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10679-1-AP (same clone as 10679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10679-1-AP (same clone as 10679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10679-1-AP (same clone as 10679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10679-1-AP (same clone as 10679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

• 

  IF Staining of LNCaP using 10679-1-AP (same clone as 10679-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using KLK3/PSA antibody (10679-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10679-1-PBS in a different storage buffer formulation.

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Product Information

10679-1-PBS targets KLK3/PSA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLK3/PSA fusion protein Ag1060
• Full Name: kallikrein-related peptidase 3
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC005307
• Gene Symbol: KLK3
• Gene ID (NCBI): 354
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms  produced by alternative splicing.