KLK3/PSA Polyclonal antibody

KLK3/PSA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 10679-1-AP

Synonyms

KLK3, KLK2A1, Kallikrein-3, Kallikrein 3, Gamma-seminoprotein

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Validation Data Gallery

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  WB analysis using 10679-1-AP

  DU 145(PSA-), PC-3(PSA-) and LNCaP (PSA+) cell lysates were subjected to SDS PAGE followed by western blot with 10679-1-AP (KLK3/PSA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 10679-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10679-1-AP (KLK3/PSA antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of LNCaP using 10679-1-AP

  IP result of anti-KLK3/PSA (IP:10679-1-AP, 4ug; Detection:10679-1-AP 1:10000) with LNCaP cells lysate 1520 ug.

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of LNCaP using 10679-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using KLK3/PSA antibody (10679-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, Transfected HEK-293 cells
• Positive IP detected in: LNCaP cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 18 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

10679-1-AP targets KLK3/PSA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLK3/PSA fusion protein Ag1060
• Full Name: kallikrein-related peptidase 3
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC005307
• Gene Symbol: KLK3
• Gene ID (NCBI): 354
• RRID: AB_2134244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms  produced by alternative splicing.

Publications

Species	Application	Title
human	IHC	Theranostics; ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3.; Authors - Lijie Zhou
mouse	IHC	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB	J Transl Med; CAPN2 promotes apalutamide resistance in metastatic hormone-sensitive prostate cancer by activating protective autophagy; Authors - Zihao Qi
human	WB	Elife; A feedback loop between the androgen receptor and 6-phosphogluoconate dehydrogenase (6PGD) drives prostate cancer growth; Authors - Joanna L Gillis
human	WB	Mol Cancer Ther; PMID: 28802253; Authors - Linh N K Tran
mouse	WB,IF	Stem Cell Res Ther; Expansion of mouse castration-resistant intermediate prostate stem cells in vitro; Authors - Yalan Xu