KLF6 Monoclonal antibody

KLF6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2F8B3

Cat no : 67297-1-Ig

Synonyms

B cell derived protein 1, COPEB, CPBP, DKFZp686N0199, GBF, KLF6, Krueppel like factor 6, Kruppel like factor 6, PAC1, Proto oncogene BCD1, ST12, Transcription factor Zf9, ZF9

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Validation Data Gallery

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  WB analysis using 67297-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67297-1-Ig (KLF6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: JAR cells, NCCIT cells, L02 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

67297-1-Ig targets KLF6 in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KLF6 fusion protein Ag6859
• Full Name: Kruppel-like factor 6
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32-42 kDa
• GenBank Accession Number: BC000311
• Gene Symbol: KLF6
• Gene ID (NCBI): 1316
• RRID: AB_2882561
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLF6(krupple-like factor 6) is a zinc finger transcription factor and tumor suppressor with biological activities and transcriptional targets in growing range. It is highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. One key target gene of KLF6 in endoglin, which is a homodimeric cell membrane glycoprotein and TGF-β auxiliary receptor, owns a pro-angiogenic role in endothelial cells and is also involved in malignant progression. Also KLF6 induces apoptosis in prostate cancer cells through upregulation of ATF3. KLF6 was normal detected as a 46kDa protein, which is larger than translation product(32kDa), because of post-translational modification including both phosphorylation and glycosylation. This antibody raise against the full length of KLF6 gene of human origin, and can recognize both of KLF6, included KLF6(~32kDa), p-KLF6(~35-46kDa). Otherwise, there may be a non-specific band(50kDa) showed in detection

Publications

Species	Application	Title
human	WB	BMC Mol Cell Biol; LncRNA LUCAT1/miR-181a-5p axis promotes proliferation and invasion of breast cancer via targeting KLF6 and KLF15.; Authors - Yun Liu
mouse,human	WB	Cell Signal; Mammalian cleavage factor 25 targets KLF14 to inhibit hepatic stellate cell activation and liver fibrosis; Authors - Xiaoyan Chen
mouse	WB	Life Sci; The zinc-finger transcription factor KLF6 regulates cardiac fibrosis; Authors - Nan Li