KLF5 Recombinant antibody, PBS Only

KLF5 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230509G7

Cat no : 83205-4-PBS

Synonyms

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

---

Validation Data Gallery

• 

  IF Staining of HeLa using 83205-4-RR (same clone as 83205-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KLF5 antibody (83205-4-RR, Clone: 230509G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83205-4-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 83205-4-RR (same clone as 83205-4-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Klf5 Recombinant Antibody (83205-4-RR, Clone:230509G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83205-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HT-29 using 83205-4-RR (same clone as 83205-4-PBS)

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Klf5 Recombinant Antibody (83205-4-RR, Clone:230509G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83205-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83205-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83205-4-RR against Human KLF5 were performed. The affinity constant is 2.36 nM.

---

Product Information

The immunogen of 83205-4-PBS is KLF5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KLF5 fusion protein Ag15267
• Full Name: Kruppel-like factor 5 (intestinal)
• Calculated Molecular Weight: 457 aa, 51 kDa
• GenBank Accession Number: BC042131
• Gene Symbol: KLF5
• Gene ID (NCBI): 688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.