KLF5 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

KLF5 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

2C11C5

Cat no : 66850-1-PBS

Synonyms

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

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Validation Data Gallery

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  WB analysis of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

  WB result of KLF5 antibody (66850-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KLF5 transfected HeLa cells. This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

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  WB analysis using 66850-1-Ig (same clone as 66850-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66850-1-Ig (KLF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human KLF5 (66850-1-Ig, Clone:2C11C5) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

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Product Information

66850-1-PBS targets KLF5 in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

• Tested Reactivity: mouse, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KLF5 fusion protein Ag15403
• Full Name: Kruppel-like factor 5 (intestinal)
• Calculated Molecular Weight: 457 aa, 51 kDa
• Observed Molecular Weight: 51-57 kDa
• GenBank Accession Number: BC042131
• Gene Symbol: KLF5
• Gene ID (NCBI): 688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.