KLF15 Monoclonal antibody, PBS Only

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  WB analysis of human skeletal muscle using 66185-1-Ig (same clone as 66185-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66185-1-Ig (KLF15 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66185-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66185-1-Ig (same clone as 66185-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66185-1-Ig (KLF15 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66185-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66185-1-Ig (same clone as 66185-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KLF15 antibody (66185-1-Ig, Clone: 2B8G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66185-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

KLF15 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 66185-1-PBS

Clone No.2B8G9

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

DKFZp779M1320, KKLF, KLF15, Krueppel like factor 15, Kruppel like factor 15

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Product Information

66185-1-PBS targets KLF15 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: KLF15 fusion protein Ag4864
• Full Name: Kruppel-like factor 15
• Calculated Molecular Weight: 416 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC036733
• Gene Symbol: KLF15
• Gene ID (NCBI): 28999
• RRID: AB_2881580
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UIH9
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

KLF15, a kidney-enriched Krüppel-like zinc finger-containing transcription factors, is a transcriptional activator that binds the CLCNKA promoter. The nuclear protein KLF15, is expressed primarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. It is an important regulator of GLUT4 in both adipose and muscle tissues.(PMID:10982849,PMID:16615998).