KIR2DL4/CD158d Polyclonal antibody

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  WB analysis using 32059-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32059-1-AP (KIR2DL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

KIR2DL4/CD158d Polyclonal Antibody for WB, ELISA

Cat No. 32059-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

KIR2DL4, KIR103, KIR 103AS, Killer cell immunoglobulin-like receptor 2DL4, G9P

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Tested Applications

• Positive WB detected in: HL-60 cells, NK-92 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32059-1-AP targets KIR2DL4/CD158d in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4
• Calculated Molecular Weight: 377 aa, 42 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC041611
• Gene Symbol: KIR2DL4
• Gene ID (NCBI): 3805
• RRID: AB_3670180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q99706
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells that regulate their responsiveness to signals received from their environment (PMID: 22934097). Killer cell immunoglobulin-like receptor 2DL4 (KIR2DL4, also known as CD158D and KIR103AS) is an unusual member of the KIRs family, which activates the cytotoxicity of NK cells. KIR2DL4 has two immunoglobulin domains and a single immunoreceptor tyrosine-based inhibition motif (ITIM) in the cytoplasmic tail with weak inhibitory potential (PMID: 12055234).