KIF5A Monoclonal antibody, PBS Only

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  WB analysis using 67009-1-Ig (same clone as 67009-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67009-1-Ig (KIF5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67009-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67009-1-Ig (same clone as 67009-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67009-1-Ig (KIF5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67009-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-12 using 67009-1-Ig (same clone as 67009-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (67009-1-Ig, Clone: 1A7E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67009-1-PBS in a different storage buffer formulation.

KIF5A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67009-1-PBS

Clone No.1A7E3

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

D12S1889, KIF5A, kinesin family member 5A, Kinesin heavy chain isoform 5A, MY050, Neuronal kinesin heavy chain, NKHC, NKHC1, SPG10

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Product Information

67009-1-PBS targets KIF5A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KIF5A fusion protein Ag15682
• Full Name: kinesin family member 5A
• Calculated Molecular Weight: 1032 aa, 117 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC146670
• Gene Symbol: KIF5A
• Gene ID (NCBI): 3798
• RRID: AB_2882326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q12840
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.