KIF5A Monoclonal antibody

KIF5A Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A7E3

Cat no : 67009-1-Ig

Synonyms

D12S1889, KIF5A, kinesin family member 5A, Kinesin heavy chain isoform 5A, MY050, Neuronal kinesin heavy chain, NKHC, NKHC1, SPG10

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Validation Data Gallery

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  WB analysis using 67009-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67009-1-Ig (KIF5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67009-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67009-1-Ig (KIF5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-12 using 67009-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (67009-1-Ig, Clone: 1A7E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat brain tissue, pig brain tissue, rat cerebellum tissue, mouse brain tissue
• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67009-1-Ig targets KIF5A in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KIF5A fusion protein Ag15682
• Full Name: kinesin family member 5A
• Calculated Molecular Weight: 1032 aa, 117 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC146670
• Gene Symbol: KIF5A
• Gene ID (NCBI): 3798
• RRID: AB_2882326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.