KIF3A Polyclonal antibody

• Featured Product
• KD/KO Validated

KIF3A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 13930-1-AP

Synonyms

KIF3, KIF3A, kinesin family member 3A, Kinesin like protein KIF3A

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Validation Data Gallery

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  WB analysis of mouse brain using 13930-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13930-1-AP (KIF3A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 13930-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13930-1-AP (KIF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 13930-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13930-1-AP (KIF3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13930-1-AP

  IP result of anti-KIF3A (IP:13930-1-AP, 4ug; Detection:13930-1-AP 1:500) with mouse brain tissue lysate 8000ug.

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  IP experiment using 13930-1-AP

  WB and IP result of the antibdoy anti-KIF3A (13930-1-AP) in mouse cell from Corbit, Kevin.

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  IHC staining of human gliomas using 13930-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13930-1-AP (KIF3A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 13930-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13930-1-AP (KIF3A antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human cerebellum tissue, mouse cerebellum tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 13 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

13930-1-AP targets KIF3A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF3A fusion protein Ag4954
• Full Name: kinesin family member 3A
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC045542
• Gene Symbol: KIF3A
• Gene ID (NCBI): 11127
• RRID: AB_2132063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF3A is a subunit of Kinesin II that is essential for intraflagellar transport (IFT). It functions as the anterograde motor to deliver ciliary components to the tip where the axoneme is assembled. Disruption of KIF3A has been reported to block mammalian ciliogenesis. Recently KIF3A has been found to localize to the mother centriole and is required for centrioles organization. This antibody specifically recognizes the endogenous KIF3A protein in mouse embryonic fibroblasts (MEFs) but not in KIF3A-/- lysate. (23386061)

Publications

Species	Application	Title
human	WB	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
human	WB	Sci Adv; An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.; Authors - Molly M Wilson
human		Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
human	WB	Proc Natl Acad Sci U S A; EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.; Authors - Vincent J Guen ; KO Validated
mouse, human	WB,IF	EMBO J; Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages.; Authors - Kodani Andrew A ; KD Validated
human	WB,IF	Cell Mol Life Sci; Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.; Authors - Delowar Hossain ; KD Validated