CoraLite® Plus 488-conjugated KIF2A Polyclonal antibody

• Featured Product
• KD/KO Validated

KIF2A Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-27218

Synonyms

HK2, KIF2, KIF2A, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A, KNS2

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Validation Data Gallery

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  IF Staining of A549 using CL488-27218

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 KIF2A antibody (CL488-27218) at dilution of 1:200.

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  IF Staining of HeLa using CL488-27218

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 KIF2A antibody (CL488-27218) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: A549 cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-27218 targets KIF2A in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF2A fusion protein Ag25692
• Full Name: kinesin heavy chain member 2A
• Calculated Molecular Weight: 679 aa, 77 kDa
• Observed Molecular Weight: 80 kDa~100 kDa
• GenBank Accession Number: BC031828
• Gene Symbol: KIF2A
• Gene ID (NCBI): 3796
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Kinesin Family Member 2A (KIF2A), also known as Kinesin-2, is a member of the kinesin-13 superfamily protein. KIF2A is a microtubule-depolymerizing kinesin motor protein with essential roles in neural progenitor division and axonal pruning during brain development. KIF2A is involved in microtubule depolymerization at minus ends of the spindle to generate pole-migration forces for chromosome movement. KIF2A is a crucial factor in the invasion and metastasis of various tumors, such as gastric cancer(PMID: 32801897), hepatocellular carcinoma(PMID: 36277155).