KIF2A Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

KIF2A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13105-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis of HeLa using 13105-1-AP (same clone as 13105-1-PBS)

  WB result of KIF2A antibody (13105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF2A transfected HeLa cells. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13105-1-AP (same clone as 13105-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13105-1-AP (KIF2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 13105-1-AP (same clone as 13105-1-PBS)

  IP result of anti-KIF2A (IP:13105-1-AP, 4ug; Detection:13105-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 13105-1-AP (same clone as 13105-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using KIF2A antibody (13105-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MDCK using 13105-1-AP (same clone as 13105-1-PBS)

  Immunofluorescent analysis of MDCK cells using 13105-1-AP (KIF2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 13105-1-AP (same clone as 13105-1-PBS)

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KIF2A (13105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

---

Product Information

13105-1-PBS targets KIF2A in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF2A fusion protein Ag3748
• Full Name: kinesin heavy chain member 2A
• Calculated Molecular Weight: 679 aa, 77 kDa
• Observed Molecular Weight: 80-100 kDa
• GenBank Accession Number: BC031828
• Gene Symbol: KIF2A
• Gene ID (NCBI): 3796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.