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- KD/KO Validated
KIF2A Polyclonal antibody
KIF2A Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, Canine
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13105-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MDCK cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 7 publications below |
IF | See 7 publications below |
Product Information
13105-1-AP targets KIF2A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
Tested Reactivity | human, mouse, rat, Canine |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | KIF2A fusion protein Ag3748 |
Full Name | kinesin heavy chain member 2A |
Calculated molecular weight | 679 aa, 77 kDa |
Observed molecular weight | 80-100 kDa |
GenBank accession number | BC031828 |
Gene symbol | KIF2A |
Gene ID (NCBI) | 3796 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for KIF2A antibody 13105-1-AP | Download protocol |
IHC protocol for KIF2A antibody 13105-1-AP | Download protocol |
IF protocol for KIF2A antibody 13105-1-AP | Download protocol |
IP protocol for KIF2A antibody 13105-1-AP | Download protocol |
FC protocol for KIF2A antibody 13105-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cell Biol WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin. | ||
EMBO Rep Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis | ||
Front Oncol Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma. | ||
Biomed Pharmacother KIF2A promotes the progression via AKT signaling pathway and is upregulated by transcription factor ETV4 in human gastric cancer.
| ||
Mol Biol Cell Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment. | ||
Biochem Biophys Res Commun High KIF2A expression promotes proliferation, migration and predicts poor prognosis in lung adenocarcinoma.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Dorota (Verified Customer) (04-10-2019) | Good for western blotting.
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