KIF2A Polyclonal antibody

• Featured Product
• KD/KO Validated

KIF2A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 13105-1-AP

Synonyms

HK2, KIF2, KIF2A, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A, KNS2

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Validation Data Gallery

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  WB analysis of HeLa using 13105-1-AP

  WB result of KIF2A antibody (13105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF2A transfected HeLa cells.

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  WB analysis using 13105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13105-1-AP (KIF2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13105-1-AP

  IP Result of anti-KIF2A (IP:13105-1-AP, 4ug; Detection:13105-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 13105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 13105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MDCK using 13105-1-AP

  Immunofluorescent analysis of MDCK cells using 13105-1-AP (KIF2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 7 publications below

Product Information

13105-1-AP targets KIF2A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF2A fusion protein Ag3748
• Full Name: kinesin heavy chain member 2A
• Calculated molecular weight: 679 aa, 77 kDa
• Observed molecular weight: 80-100 kDa
• GenBank accession number: BC031828
• Gene symbol: KIF2A
• Gene ID (NCBI): 3796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IF	J Cell Biol; WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.; Authors - Junjie Huang
mouse	WB,IF	EMBO Rep; Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis; Authors - Jia-Qian Ju
human	WB	Front Oncol; Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma.; Authors - Xigang Xia
human	WB	Biomed Pharmacother; KIF2A promotes the progression via AKT signaling pathway and is upregulated by transcription factor ETV4 in human gastric cancer.; Authors - Xin Zhang ; KD Validated
human	IF	Mol Biol Cell; Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment.; Authors - Hongdan Yang
human	WB	Biochem Biophys Res Commun; High KIF2A expression promotes proliferation, migration and predicts poor prognosis in lung adenocarcinoma.; Authors - Tian Xie ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Dorota (Verified Customer) (04-10-2019)

Good for western blotting.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250 -1:1000
• Cell Tissue Type: U2OS

KIF2A Antibody Western Blot, validation (1:250 -1:1000 dilution) in U2OS (Cat no:13105-1-AP)