ATG13 Monoclonal antibody

ATG13 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1C3A7

Cat no : 66708-1-Ig

Synonyms

KIAA0652, Autophagy-related protein 13, Autophagy related protein 13, 1C3A7

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Validation Data Gallery

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  WB analysis using 66708-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66708-1-Ig (ATG13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66708-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of BGC-823 using 66708-1-Ig

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66708-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 66708-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66708-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 66708-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66708-1-Ig (KIAA0652 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 66708-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66708-1-Ig (KIAA0652 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66708-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66708-1-Ig (KIAA0652 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66708-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66708-1-Ig (KIAA0652 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66708-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATG13 antibody (66708-1-Ig, Clone: 1C3A7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 66708-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATG13 (66708-1-Ig, Clone:1C3A7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, BGC-823 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells, Jurkat cells
• Positive IHC detected in: mouse testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

66708-1-Ig targets ATG13 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATG13 fusion protein Ag12968
• Full Name: KIAA0652
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC001331
• Gene Symbol: ATG13
• Gene ID (NCBI): 9776
• RRID: AB_2882060
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

Publications

Species	Application	Title
mouse	WB	Cell Rep; Destabilization of TP53 by USP10 is essential for neonatal autophagy and survival; Authors - Hongchang Li
human	WB	Cancer Chemother Pharmacol; Avermectin B1 mediates antitumor activity and induces autophagy in osteosarcoma through the AMPK/ULK1 signaling pathway; Authors - Xiang Fei

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C