ATG13 Monoclonal antibody, PBS Only

ATG13 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

pig, rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C3A7

Cat no : 66708-1-PBS

Synonyms

KIAA0652, Autophagy-related protein 13, Autophagy related protein 13, 1C3A7

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Validation Data Gallery

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  WB analysis using 66708-1-Ig (same clone as 66708-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66708-1-Ig (ATG13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

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  WB analysis of BGC-823 using 66708-1-Ig (same clone as 66708-1-PBS)

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66708-1-Ig (same clone as 66708-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66708-1-Ig (same clone as 66708-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66708-1-Ig (same clone as 66708-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66708-1-Ig (same clone as 66708-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66708-1-Ig (same clone as 66708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66708-1-Ig (KIAA0652 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66708-1-Ig (same clone as 66708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66708-1-Ig (KIAA0652 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66708-1-Ig (same clone as 66708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66708-1-Ig (KIAA0652 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66708-1-Ig (same clone as 66708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66708-1-Ig (KIAA0652 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66708-1-Ig (same clone as 66708-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATG13 antibody (66708-1-Ig, Clone: 1C3A7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66708-1-Ig (same clone as 66708-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATG13 (66708-1-Ig, Clone:1C3A7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

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Product Information

66708-1-PBS targets ATG13 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with pig, rat, mouse, human samples.

• Tested Reactivity: pig, rat, mouse, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATG13 fusion protein Ag12968
• Full Name: KIAA0652
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC001331
• Gene Symbol: ATG13
• Gene ID (NCBI): 9776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)