KHSRP Recombinant antibody

KHSRP Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 82931-1-RR
Clone No.230189G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, Jurkat cells, mouse spleen tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82931-1-RR targets KHSRP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KHSRP fusion protein Ag33890 Predict reactive species
Full Name KH-type splicing regulatory protein
Calculated Molecular Weight73 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberNM_003685
Gene Symbol KHSRP
Gene ID (NCBI) 8570
RRIDAB_3670669
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possi3bly by recruiting degradation machinery to ARE-containing mRNAs.

Protocols

Product Specific Protocols
WB protocol for KHSRP antibody 82931-1-RRDownload protocol
IHC protocol for KHSRP antibody 82931-1-RRDownload protocol
IF protocol for KHSRP antibody 82931-1-RRDownload protocol
IP protocol for KHSRP antibody 82931-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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