Various lysates were subjected to SDS PAGE followed by western blot with 82931-1-RR (KHSRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82931-1-RR (KHSRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 82931-1-RR
IP result of anti-KHSRP (IP:82931-1-RR, 4ug; Detection:82931-1-RR 1:2500) with HeLa cells lysate 1050 ug.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82931-1-RR (KHSRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 82931-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (82931-1-RR, Clone: 230189G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (82931-1-RR, Clone: 230189G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of HeLa using 82931-1-RR
1x10^6 HeLa cells were intracellularly stained with 0.4 ug KHSRP Recombinant antibody (82931-1-RR, Clone:230189G1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1x10^6 HeLa cells were intracellularly stained with 0.4 ug KHSRP Recombinant antibody (82931-1-RR, Clone:230189G1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
Affinity and Kinetic Characterization of 82931-1-RR
Biolayer interferometry (BLl) kinetic assays of 82931-1-RR against Human KHSRP were performed. The affinity constant is 0.597 nM.
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HeLa cells, HEK-293T cells, Jurkat cells, mouse spleen tissue, mouse brain tissue, rat brain tissue
Positive IP detected in
HeLa cells
Positive IHC detected in
mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Positive FC (Intra) detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
82931-1-RR targets KHSRP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possi3bly by recruiting degradation machinery to ARE-containing mRNAs.
Various lysates were subjected to SDS PAGE followed by western blot with 82931-1-RR (KHSRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 82931-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82931-1-RR (KHSRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using 82931-1-RR
IP result of anti-KHSRP (IP:82931-1-RR, 4ug; Detection:82931-1-RR 1:2500) with HeLa cells lysate 1050 ug.
IF/ICC Figures
IF Staining of HeLa using 82931-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (82931-1-RR, Clone: 230189G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of HeLa using 82931-1-RR
1x10^6 HeLa cells were intracellularly stained with 0.4 ug KHSRP Recombinant antibody (82931-1-RR, Clone:230189G1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
AFFINITY Figures
Affinity and Kinetic Characterization of 82931-1-RR
Biolayer interferometry (BLl) kinetic assays of 82931-1-RR against Human KHSRP were performed. The affinity constant is 0.597 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KHSRP Recombinant antibody
Catalog Number
82931-1-RR
Citations
-
Dilutions
WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
