KEAP1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

KEAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgA

Reactivity

human, rat, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G10H9

Cat no : 60027-1-PBS

Synonyms

Cytosolic inhibitor of Nrf2, INrf2, KEAP1, Kelch like protein 19, KIAA0132, KLHL19

---

Validation Data Gallery

• 

  WB analysis of 4T1 using 60027-1-Ig (same clone as 60027-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 60027-1-Ig (KEAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60027-1-Ig (same clone as 60027-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60027-1-Ig (KEAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60027-1-Ig (same clone as 60027-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60027-1-Ig (KEAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 60027-1-Ig (same clone as 60027-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60027-1-Ig (KEAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60027-1-Ig (same clone as 60027-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60027-1-Ig (KEAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 60027-1-Ig (same clone as 60027-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60027-1-Ig (KEAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 60027-1-Ig (same clone as 60027-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60027-1-Ig (KEAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 60027-1-Ig (same clone as 60027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60027-1-Ig(KEAP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 60027-1-Ig (same clone as 60027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 60027-1-Ig(KEAP1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 60027-1-Ig (same clone as 60027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 60027-1-Ig(KEAP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 60027-1-Ig (same clone as 60027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60027-1-Ig(KEAP1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60027-1-Ig (same clone as 60027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60027-1-Ig(KEAP1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60027-1-Ig (same clone as 60027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60027-1-Ig(KEAP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60027-1-Ig (same clone as 60027-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KEAP1 antibody (60027-1-Ig, Clone: 4G10H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

---

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Product Information

60027-1-PBS targets KEAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgA
• Class: Monoclonal
• Type: Antibody
• Immunogen: KEAP1 fusion protein Ag0779
• Full Name: kelch-like ECH-associated protein 1
• Calculated Molecular Weight: 624 aa, 70 kDa
• Observed Molecular Weight: 60~70 kDa
• GenBank Accession Number: BC002930
• Gene Symbol: KEAP1
• Gene ID (NCBI): 9817
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Kelch-like ECH-associated protein 1 (KEAP1) is a negative regulator of nuclear factor erythroid 2-related factor 2 (Nrf2), a transcription factor governing the antioxidant response.

What is the molecular weight of KEAP1 protein? Are there any isoforms of KEAP1?

The molecular weight of KEAP1 protein is 70 kDa. The KEAP1 gene gives rise only to protein isoforms, but mutations of KEAP1 protein have been found in various cancer types.

What is the subcellular localization of KEAP1?

KEAP1 resides in the cytoplasm, where it binds to Nrf2, targeting it for degradation and preventing translocation of Nrf2 to the nucleus.

How does KEAP1 control Nrf2 levels? Is KEAP1 post-translationally modified?

KEAP1 is rich in reactive cysteine residues, whose thiol groups play a role in binding to CUL3 and the polyubiquitination of Nrf2, which leads to degradation of Nrf2 via the proteasome system. During oxidative stress, electrophiles and reactive oxygen species (ROS) modify the KEAP1 thiol groups, reducing the affinity of KEAP1 to CUL3 and the stabilization of Nrf2. Nrf2 then translocates to the nucleus, where it binds to the antioxidant responsive elements (AREs) and induces the expression of antioxidant proteins (PMID: 16354693).

How to measure oxidative stress using KEAP1 and Nrf2 proteins as a readout

Under basal conditions (unstressed cells), a detectable KEAP1 protein level is observed. Oxidative stress modifies KEAP1 protein activity by increasing the Nrf2 protein levels. This can be measured, for example, using western blotting (PMID: 27697860). KEAP1 protein levels are not altered by oxidative stress.

What is the role of the KEAP1-Nrf2 pathway in health and disease?

The KEAP-Nrf2 pathway plays a vital role in redox homeostasis and cryoprotection. Inhibition of KEAP1 activity leads to the activation of Nrf2 and increase the response to oxidative stress and anti-inflammatory effects (PMID: 29717933). The activation of Nrf2 can be beneficial in the case of metabolic diseases, such as diabetes, as well as neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. However, the increased activation of Nrf2 is also known to promote tumor growth and metastasis. Mutations in both KEAP1 and Nrf2 were found in various solid tumor types.