VEGFR2/KDR Recombinant antibody, PBS Only (Capture)

VEGFR2/KDR Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230238D2

Cat no : 83049-2-PBS

Synonyms

KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00012-3

  Sandwich ELISA standard curve of MP00012-3, Human VEGFR2/KDR Recombinant Matched Antibody Pair - PBS only. 83049-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0218.83049-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP00012-3

  HUVEC was cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for human VEGFR2/KDR, and measured 267.3 pg/mL.

• 

  Sample test of MP00012-3

  The mean human VEGFR2/KDR concentration was determined to be 55,216.6 pg/mL in HUVEC cell extract based on a 0.4 mg/mL extract load.

• 

  Sample test of MP00012-3

  Plasma of eight individual healthy human donors was measured. The human VEGFR2/KDR concentration of detected samples was determined to be 5,643.5 ng/mL with a range of 1,988.2 - 9,946.0 pg/mL.

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  Cytometric bead array standard curve of MP00012-1

  Cytometric bead array standard curve of MP00012-1, KDR Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83049-2-PBS. Detection antibody: 83049-3-PBS. Standard: Eg0218. Range: 0.312-20 ng/mL.

• 

  Cytometric bead array standard curve of MP00012-2

  Cytometric bead array standard curve of MP00012-2, KDR Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83049-2-PBS. Detection antibody: 83049-1-PBS. Standard: Eg0218. Range: 0.625-20 ng/mL.

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Product Information

83049-2-PBS targets VEGFR2/KDR as part of a matched antibody pair:

MP00012-1: 83049-2-PBS capture and 83049-3-PBS detection (validated in Cytometric bead array)

MP00012-2: 83049-2-PBS capture and 83049-1-PBS detection (validated in Cytometric bead array)

MP00012-3: 83049-2-PBS capture and 83049-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Affinity:
  K_D=1.15 x 10^-9M
  K_Off=2.30 x 10^-4M
  K_On=2.00 x 10^5M
• Immunogen: Recombinant protein
• Full Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Calculated Molecular Weight: 152kd
• GenBank Accession Number: NM_002253
• Gene Symbol: KDR
• Gene ID (NCBI): 3791
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.