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KDM3A/JMJD1A Recombinant antibody, PBS Only

KDM3A/JMJD1A Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240532E2

Cat no : 83571-6-PBS

Synonyms

KDM3A, KDM3A,JMJD1A, JmjC domain-containing histone demethylation protein 2A, JHMD2A, JHDM2A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KDM3A/JMJD1A Recombinant antibody (83571-6-RR)

    IF Staining of HepG2 using 83571-6-RR (same clone as 83571-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KDM3A,JMJD1A antibody (83571-6-RR, Clone: 240532E2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83571-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using KDM3A/JMJD1A Recombinant antibody (83571-6-RR)

    FC experiment of HEK-293 using 83571-6-RR (same clone as 83571-6-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug KDM3A,JMJD1A Recombinant antibody (83571-6-RR, Clone:240532E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83571-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83571-6-RR

    Affinity and Kinetic Characterization of 83571-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83571-6-RR against Human KDM3A,JMJD1A were performed. The affinity constant is 6.71 nM.

Product Information

The immunogen of 83571-6-PBS is KDM3A/JMJD1A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KDM3A/JMJD1A fusion protein Ag28687
Full Name lysine (K)-specific demethylase 3A
Calculated Molecular Weight 150 kDa
GenBank Accession NumberBC038297
Gene Symbol KDM3A/JMJD1A
Gene ID (NCBI) 55818
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC.