KDM3A/JMJD1A Recombinant antibody, PBS Only (Capture)

KDM3A/JMJD1A Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240532G11

Cat no : 83571-2-PBS

Synonyms

KDM3A, KDM3A,JMJD1A, JmjC domain-containing histone demethylation protein 2A, JHMD2A, JHDM2A

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Validation Data Gallery

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  WB analysis using 83571-2-RR (same clone as 83571-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83571-2-RR (KDM3A,JMJD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83571-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 83571-2-RR (same clone as 83571-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83571-2-RR (KDM3A,JMJD1A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83571-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83571-2-RR (same clone as 83571-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KDM3A,JMJD1A antibody (83571-2-RR, Clone: 240532G11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83571-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 83571-2-RR (same clone as 83571-2-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug KDM3A,JMJD1A Recombinant antibody (83571-2-RR, Clone:240532G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83571-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00565-1

  Cytometric bead array standard curve of MP00565-1, KDM3A,JMJD1A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83571-2-PBS. Detection antibody: 83571-4-PBS. Standard: Ag28687. Range: 1.56-100 ng/mL.

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  Cytometric bead array standard curve of MP00565-2

  Cytometric bead array standard curve of MP00565-2, KDM3A,JMJD1A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83571-2-PBS. Detection antibody: 83571-5-PBS. Standard: Ag28687. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 83571-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83571-2-RR against Human KDM3A,JMJD1A were performed. The affinity constant is below 1 pM.

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Product Information

83571-2-PBS targets KDM3A/JMJD1A as part of a matched antibody pair:

MP00565-1: 83571-2-PBS capture and 83571-4-PBS detection (validated in Cytometric bead array)

MP00565-2: 83571-2-PBS capture and 83571-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KDM3A/JMJD1A fusion protein Ag28687
• Full Name: lysine (K)-specific demethylase 3A
• Calculated Molecular Weight: 150 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC038297
• Gene Symbol: KDM3A/JMJD1A
• Gene ID (NCBI): 55818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC.