CoraLite® Plus 488-conjugated KCNN4 Polyclonal antibody

• Featured Product
• KD/KO Validated

KCNN4 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-23271

Synonyms

hIKCa1, hKCa4, hSK4, IK1, IKCA1, KCa3.1, KCA4, KCNN4, Putative Gardos channel, SK4, SKCa 4, SKCa4

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-23271

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 KCNN4 antibody (CL488-23271) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-23271 targets KCNN4 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KCNN4 fusion protein Ag19774
• Full Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4
• Calculated Molecular Weight: 427 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC015337
• Gene Symbol: KCNN4
• Gene ID (NCBI): 3783
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The intermediate conductance calcium-activated potassium channel KCNN4, also known as KCa3.1, IK1, or SK4, is a member of the potassium channel KCNN family. KCNN4 is expressed in T cells, mast cells, macrophages, erythrocytes, vascular smooth muscle cells, airway smooth muscle cells, intestinal and airway epithelial cells (PMID: 19681865; 21493782). Functionally, KCNN4 plays a key role in calcium-dependent cell functions, such as proliferation, activation, and migration, in a broad range of cell types (PMID: 21493782).