KBTBD8 Polyclonal antibody

• 

  WB analysis using 31970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31970-1-AP (KBTBD8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

KBTBD8 Polyclonal Antibody for WB, ELISA

Cat No. 31970-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

T-cell activation kelch repeat protein, TA-KRP, TAKRP, KIAA1842, Kelch repeat and BTB domain-containing protein 8

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Tested Applications

• Positive WB detected in: Daudi cells, RKO cells, U-118 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31970-1-AP targets KBTBD8 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KBTBD8 fusion protein Ag36219
• Full Name: kelch repeat and BTB (POZ) domain containing 8
• Calculated Molecular Weight: 69 kDa，601aa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_032505.2
• Gene Symbol: KBTBD8
• Gene ID (NCBI): 84541
• RRID: AB_3670161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8NFY9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kelch repeat and BTB domain containing 8 (KBTBD8, also known as TAKRP and TA-KRP) is a member of the BTB-kelch family of proteins, which is specifically localized at the Golgi apparatus in nondividing cells and becomes restricted to the spindle apparatus upon mitosis (PMID: 23578279). KBTBD family proteins are involved in ubiquitination in various cells and tissues and are correlated with human muscle diseases (PMID: 9878064; 16547521; 24959344). The expression level of KBTBD8 might affect the proliferation and migration of epithelial ovarian cancer (PMID: 33109073).