TIP60/KAT5 Recombinant antibody

TIP60/KAT5 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230181D5

Cat no : 82937-1-RR

Synonyms

KAT5, EC:2.3.1.48, EC:2.3.1.-, cPLA2, cPLA(2)-interacting protein

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Validation Data Gallery

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  IF Staining of HepG2 using 82937-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAT5 antibody (82937-1-RR, Clone: 230181D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of HCT 116 using 82937-1-RR

  1x10^6 HCT 116 cells were intracellularly stained with 0.25 ug Anti-Human KAT5 (82937-1-RR, Clone:230181D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 82937-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82937-1-RR against Human TIP60/KAT5 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HCT 116 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82937-1-RR targets TIP60/KAT5 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TIP60/KAT5 fusion protein Ag1261
• Full Name: K(lysine) acetyltransferase 5
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: BC000166
• Gene Symbol: KAT5
• Gene ID (NCBI): 10524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KAT5, also named as The histone acetyltransferase (HAT) Tat interactive protein 60 kD belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis.