CoraLite® Plus 750-conjugated KAT2A/GCN5 Monoclonal antibody

• Featured Product
• KD/KO Validated

KAT2A/GCN5 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1F6G7

Cat no : CL750-66575

Synonyms

GCN5, GCN5L2, hGCN5, Histone acetyltransferase GCN5, HsGCN5, K(lysine) acetyltransferase 2A, KAT2A, Lysine acetyltransferase 2A, PCAF b, STAF97

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Validation Data Gallery

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  WB analysis using CL750-66575

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-66575 (KAT2A/GCN5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-66575 targets KAT2A/GCN5 in WB applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: KAT2A/GCN5 fusion protein Ag6940
• Full Name: K(lysine) acetyltransferase 2A
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC032743
• Gene Symbol: KAT2A
• Gene ID (NCBI): 2648
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.