KAT2A/GCN5 Polyclonal antibody, PBS Only

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  WB analysis of HUVEC using 28390-1-AP (same clone as 28390-1-PBS)

  WB result of KAT2A/GCN5 antibody (28390-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and KAT2A/GCN5 knockout HUVEC cells. This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

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  WB analysis using 28390-1-AP (same clone as 28390-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28390-1-AP (KAT2A/GCN5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

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  IP experiment of hTERT-RPE1 using 28390-1-AP (same clone as 28390-1-PBS)

  IP result of anti-KAT2A (IP:28390-1-AP, 4ug; Detection:28390-1-AP 1:400) with hTERT-RPE1 cells lysate 1645 ug. This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

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  IP experiment of LNCaP using 28390-1-AP (same clone as 28390-1-PBS)

  IP result of anti-KAT2A/GCN5 (IP:28390-1-AP, 4ug; Detection:28390-1-AP 1:6000) with LNCaP cells lysate 1200 ug. This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 28390-1-AP (same clone as 28390-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KAT2A/GCN5 antibody (28390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

KAT2A/GCN5 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 28390-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

hGCN5, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48

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Product Information

28390-1-PBS targets KAT2A/GCN5 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KAT2A/GCN5 fusion protein Ag29005
• Full Name: K(lysine) acetyltransferase 2A
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC032743
• Gene Symbol: KAT2A
• Gene ID (NCBI): 2648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92830
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.