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KAT2A/GCN5 Polyclonal antibody

KAT2A/GCN5 Polyclonal Antibody for IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 28390-1-AP

Synonyms

hGCN5, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of hTERT-RPE1 cells using KAT2A Polyclonal antibody (28390-1-AP)

    IP experiment of hTERT-RPE1 using 28390-1-AP

    IP result of anti-KAT2A (IP:28390-1-AP, 4ug; Detection:28390-1-AP 1:400) with hTERT-RPE1 cells lysate 1645 ug.

  • Immunoprecipitation (IP) experiment of LNCaP cells using KAT2A/GCN5 Polyclonal antibody (28390-1-AP)

    IP experiment of LNCaP using 28390-1-AP

    IP result of anti-KAT2A/GCN5 (IP:28390-1-AP, 4ug; Detection:28390-1-AP 1:6000) with LNCaP cells lysate 1200 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KAT2A/GCN5 Polyclonal antibody (28390-1-AP)

    IF Staining of HeLa using 28390-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KAT2A/GCN5 antibody (28390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive IP detected inLNCaP cells, hTERT-RPE1 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28390-1-AP targets KAT2A/GCN5 in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KAT2A/GCN5 fusion protein Ag29005
Full Name K(lysine) acetyltransferase 2A
Calculated Molecular Weight 94 kDa
Observed Molecular Weight94 kDa
GenBank Accession NumberBC032743
Gene Symbol KAT2A
Gene ID (NCBI) 2648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.

Protocols

Product Specific Protocols
IF protocol for KAT2A/GCN5 antibody 28390-1-APDownload protocol
IP protocol for KAT2A/GCN5 antibody 28390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols