KAP1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

KAP1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B9G12

Cat no : 66630-1-PBS

Synonyms

TRIM28, KRAB associated protein 1, KAP-1, KAP 1, EC:2.3.2.27

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Validation Data Gallery

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  WB analysis of HepG2 using 66630-1-Ig (same clone as 66630-1-PBS)

  WB result of KAP1 antibody (66630-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAP1 transfected HepG2 cells. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66630-1-Ig (same clone as 66630-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66630-1-Ig (KAP1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  WB analysis using 66630-1-Ig (same clone as 66630-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66630-1-Ig (KAP1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66630-1-Ig (same clone as 66630-1-PBS)

  IP result of anti-KAP1 (IP:66630-1-Ig, 5ug; Detection:66630-1-Ig 1:1000) with HeLa cells lysate 4000 ug. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66630-1-Ig (same clone as 66630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66630-1-Ig (same clone as 66630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66630-1-Ig (same clone as 66630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66630-1-Ig (same clone as 66630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66630-1-Ig (same clone as 66630-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (66630-1-Ig, Clone: 1B9G12 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66630-1-Ig (same clone as 66630-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (66630-1-Ig, Clone: 1B9G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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Product Information

66630-1-PBS targets KAP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: KAP1 fusion protein Ag7519
• Full Name: tripartite motif-containing 28
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC004978
• Gene Symbol: KAP1
• Gene ID (NCBI): 10155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )