KAP1 Monoclonal antibody

• Featured Product
• KD/KO Validated

KAP1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

11

CloneNo.

1B9G12

Cat no : 66630-1-Ig

Synonyms

TRIM28, KRAB associated protein 1, KAP-1, KAP 1, EC:2.3.2.27

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Validation Data Gallery

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  WB analysis of HepG2 using 66630-1-Ig

  WB result of KAP1 antibody (66630-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAP1 transfected HepG2 cells.

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  WB analysis of HeLa using 66630-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66630-1-Ig (KAP1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66630-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66630-1-Ig (KAP1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 66630-1-Ig

  IP result of anti-KAP1 (IP:66630-1-Ig, 5ug; Detection:66630-1-Ig 1:1000) with HeLa cells lysate 4000 ug.

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  IHC staining of human breast cancer using 66630-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66630-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66630-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66630-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66630-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (66630-1-Ig, Clone: 1B9G12 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 66630-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (66630-1-Ig, Clone: 1B9G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HCT 116 cells, HepG2 cells, A549 cells, HEK-293 cells, Caco-2 cells, MOLT-4 cells, TF-1 cells, RT-4 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

66630-1-Ig targets KAP1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: KAP1 fusion protein Ag7519
• Full Name: tripartite motif-containing 28
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC004978
• Gene Symbol: KAP1
• Gene ID (NCBI): 10155
• RRID: AB_2732886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )

Publications

Species	Application	Title
human	WB	Sci Adv; Epac1 activation by cAMP regulates cellular SUMOylation and promotes the formation of biomolecular condensates.; Authors - Wenli Yang
human	WB	Nucleic Acids Res; SIRT6 coordinates with CHD4 to promote chromatin relaxation and DNA repair.; Authors - Tianyun Hou
human	WB	BMC Pulm Med; miR-125b-5p upregulation by TRIM28 induces cisplatin resistance in non-small cell lung cancer through CREB1 inhibition; Authors - Qiuyu Tan ; KD Validated
human	WB	bioRxiv; Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy; Authors - Zhihua Kang
human	WB	Open Biol; A critical threshold of MCM10 is required to maintain genome stability during differentiation of induced pluripotent stem cells into natural killer cells; Authors - Megan M Schmit
human	WB	Mol Cell; CDK-independent role of D-type cyclins in regulating DNA mismatch repair; Authors - Gergely Rona