Jagged1 Monoclonal antibody

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• KD/KO Validated

Jagged1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgA

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

13

CloneNo.

2A7D11

Cat no : 66890-1-Ig

Synonyms

AGS, AHD, AWS, CD339, HJ1, JAG1, jagged 1 (Alagille syndrome), Jagged1, JAGL1, Protein jagged 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 66890-1-Ig

  WB result of Jagged1 antibody (66890-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Jagged1 transfected HEK-293 cells.

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  WB analysis of HT-29 using 66890-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66890-1-Ig (Jagged1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HT-29 cells

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• FC: See 1 publications below

Product Information

66890-1-Ig targets Jagged1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, goat
• Host / Isotype: Mouse / IgA
• Class: Monoclonal
• Type: Antibody
• Immunogen: Jagged1 fusion protein Ag11891
• Full Name: jagged 1 (Alagille syndrome)
• Calculated Molecular Weight: 1218 aa, 134 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC098393
• Gene Symbol: Jagged1
• Gene ID (NCBI): 182
• RRID: AB_2882220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Jagged1 is a type I, membrane- anchored, multidomain protein and a ligand of the Notch receptor. The receptor-ligand interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis. The predicted MW of Jagged1 is 134 kDa, while higher MW around 150-180 kDa had been observed in literations (PMID: 9716576, 9596653).

Publications

Species	Application	Title
human	WB	Int J Biol Sci; Linc-KILH potentiates Notch1 signaling through inhibiting KRT19 phosphorylation and promotes the malignancy of hepatocellular carcinoma.; Authors - Xudong Zhang
human	FC	Commun Biol; Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells.; Authors - LinXi Cheng
human	WB	Front Med (Lausanne); IL-7-Treated Periodontal Ligament Cells Regulate Local Immune Homeostasis by Modulating Treg/Th17 Cell Polarization.; Authors - Xin-Yi Yu
human	WB	Cell Biochem Funct; LncRNA TDRG1/miR-214-5p axis affects preeclampsia by modulating trophoblast cells.; Authors - Fengyan Gong
human	WB	Acta Histochem; miR-214-5p suppresses the proliferation, migration and invasion of trophoblast cells in pre-eclampsia by targeting jagged 1 to inhibit notch signaling pathway.; Authors - Fengyan Gong
human	WB	Int J Med Mushrooms; Effects of Tricholoma matsutake (Agaricomycetes) Extracts on Promoting Proliferation of HaCaT Cells and Accelerating Mice Wound Healing; Authors - Wenyu Zhu