Gamma Catenin Polyclonal antibody, PBS Only

Gamma Catenin Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11146-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis of mouse heart using 11146-1-AP (same clone as 11146-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 11146-1-AP (same clone as 11146-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 11146-1-AP (same clone as 11146-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11146-1-AP (same clone as 11146-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  WB analysis of T-47D using 11146-1-AP (same clone as 11146-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HT-29 using 11146-1-AP (same clone as 11146-1-PBS)

  IP result of anti-Gamma Catenin (IP:11146-1-AP, 4ug; Detection:11146-1-AP 1:1000) with HT-29 cells lysate 1040 ug. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11146-1-AP (Gamma Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11146-1-AP (Gamma Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11146-1-AP (Gamma Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

---

Product Information

11146-1-PBS targets Gamma Catenin in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Gamma Catenin fusion protein Ag1627
• Full Name: junction plakoglobin
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC011865
• Gene Symbol: Gamma Catenin
• Gene ID (NCBI): 3728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.