JUN Monoclonal antibody, PBS Only

JUN Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G6B11

Cat no : 66313-1-PBS

Synonyms

Activator protein 1, AP 1, AP1, c Jun, JUN, jun oncogene, P39, Proto oncogene c Jun, Transcription factor AP 1

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Validation Data Gallery

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  WB analysis using 66313-1-Ig (same clone as 66313-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66313-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66313-1-Ig (same clone as 66313-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66313-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 66313-1-Ig (same clone as 66313-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66313-1-PBS in a different storage buffer formulation.

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  WB analysis of Saos-2 using 66313-1-Ig (same clone as 66313-1-PBS)

  Saos-2 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66313-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66313-1-Ig (same clone as 66313-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66313-1-PBS in a different storage buffer formulation.

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Product Information

66313-1-PBS targets JUN in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: JUN fusion protein Ag17639
• Full Name: jun oncogene
• Calculated Molecular Weight: 331 aa, 36 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC068522
• Gene Symbol: JUN
• Gene ID (NCBI): 3725
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

What is the molecular weight of JUN?

The molecular weight of JUN is approximately 39 kDa.

What is the cellular localization of JUN?

JUN expression can be located in the nucleus. However, ubiquitinated JUN colocalizes with lysosomal proteins (PMID: 15469925).

 What is the function of JUN?

JUN is a basic leucine zipper (bZIP) transcription factor that recognizes and binds to a consensus heptamer motif of 5'-TGA[CG]TCA-3' in the enhancer region of target genes. JUN acts as either a homo- or heterodimer and binds to the DNA to regulate transcriptional activity, specifically promoting the activity of NR5A1 when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon the stimulation of the cAMP signaling pathway (PMID: 9732876).

 What post-translational modifications is JUN subjected to?

JUN is acetylated at Lys-271 by EP300 (PMID: 11689449). JUN is phosphorylated by PLK3 following exposure to hypoxia or UV irradiation, which leads to increased DNA-binding activity (PMID: 27281822), and also by PAK2 at numerous threonine residues to promote cell proliferation and transformation (PMID: 21177766). JUN is also phosphorylated by DYRK2 at Ser-243, which primes it for subsequent further phosphorylation by GSK3B and reduces its ability to bind DNA (PMID: 22307329).

 What is the role of JUN in disease?

Activation of JUN is linked with proliferation and angiogenesis in invasive breast cancers (PMID: 16733206).