JNK Recombinant antibody, PBS Only

JNK Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6J16

Cat no : 81629-1-PBS

Synonyms

MAPK8, JNK1, JNK 2, JNK 1, EC:2.7.11.24

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Validation Data Gallery

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  WB analysis using 81629-1-RR (same clone as 81629-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81629-1-RR (JNK antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81629-1-RR (same clone as 81629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81629-1-RR (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81629-1-RR (same clone as 81629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81629-1-RR (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81629-1-RR (same clone as 81629-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JNK antibody (81629-1-RR, Clone: 6J16 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 81629-1-RR (same clone as 81629-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (81629-1-RR, Clone: 6J16 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 81629-1-RR (same clone as 81629-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human JNK (81629-1-RR, Clone:6J16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81629-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81629-1-RR against Human JNK were performed. The affinity constant is below 1 pM.

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Product Information

81629-1-PBS targets JNK in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: JNK fusion protein Ag1639
• Full Name: mitogen-activated protein kinase 8
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 44-48 kDa, 50-55 kDa
• GenBank Accession Number: BC130572
• Gene Symbol: JNK
• Gene ID (NCBI): 5599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has some isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ). This antibody can recognize JNK1, JNK2 and JNK3.