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JNK Recombinant antibody

JNK Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

6J16

Cat no : 81629-1-RR

Synonyms

MAPK8, JNK1, JNK 2, JNK 1, EC:2.7.11.24

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JNK Recombinant antibody (81629-1-RR)

    WB analysis using 81629-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81629-1-RR (JNK antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using JNK Recombinant antibody (81629-1-RR)

    IHC staining of human colon cancer using 81629-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81629-1-RR (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using JNK Recombinant antibody (81629-1-RR)

    IHC staining of human colon cancer using 81629-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81629-1-RR (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using JNK Recombinant antibody (81629-1-RR)

    IF Staining of Jurkat using 81629-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (81629-1-RR, Clone: 6J16 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using JNK Recombinant antibody (81629-1-RR)

    IF Staining of HeLa using 81629-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JNK antibody (81629-1-RR, Clone: 6J16 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using JNK Recombinant antibody (81629-1-RR)

    FC experiment of HeLa using 81629-1-RR

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human JNK (81629-1-RR, Clone:6J16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 81629-1-RR

    Affinity and Kinetic Characterization of 81629-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81629-1-RR against Human JNK were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, Jurkat cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81629-1-RR targets JNK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen JNK fusion protein Ag1639
Full Name mitogen-activated protein kinase 8
Calculated Molecular Weight 48 kDa
Observed Molecular Weight44-48 kDa, 50-55 kDa
GenBank Accession NumberBC130572
Gene Symbol JNK
Gene ID (NCBI) 5599
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has some isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ). This antibody can recognize JNK1, JNK2 and JNK3.

Protocols

Product Specific Protocols
WB protocol for JNK antibody 81629-1-RRDownload protocol
IHC protocol for JNK antibody 81629-1-RRDownload protocol
IF protocol for JNK antibody 81629-1-RRDownload protocol
FC protocol for JNK antibody 81629-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols