Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 66210-1-Ig (same clone as 66210-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.
WB analysis using 66210-1-Ig (same clone as 66210-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66210-1-Ig (same clone as 66210-1-PBS)
Ethacrynic acid treated HeLa cells were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.
WB analysis of RAW 264.7 using 66210-1-Ig (same clone as 66210-1-PBS)
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.
WB analysis of C6 using 66210-1-Ig (same clone as 66210-1-PBS)
C6 cells were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66210-1-Ig (same clone as 66210-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66210-1-Ig (same clone as 66210-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 66210-1-Ig (same clone as 66210-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 66210-1-Ig (same clone as 66210-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.
IF Staining of Jurkat using 66210-1-Ig (same clone as 66210-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (66210-1-Ig, Clone: 1A12E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 66210-1-Ig (same clone as 66210-1-PBS)
1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human JNK (66210-1-Ig, Clone:1A12E1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.
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1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human JNK (66210-1-Ig, Clone:1A12E1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

JNK Monoclonal antibody, PBS Only

JNK Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 66210-1-PBS

Clone No.1A12E1

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

MAPK8, JNK1, JNK 2, JNK 1, EC:2.7.11.24

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

66210-1-PBS targets JNK in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	JNK fusion protein Ag21426 Predict reactive species
Full Name	mitogen-activated protein kinase 8
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	44-48 kDa, 50-55 kDa
GenBank Accession Number	BC130572
Gene Symbol	JNK
Gene ID (NCBI)	5599
RRID	AB_2881601
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P45983
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.