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JNK Polyclonal antibody, PBS Only

JNK Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17337-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using JNK Polyclonal antibody (17337-1-AP)

    WB analysis of mouse brain using 17337-1-AP (same clone as 17337-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17337-1-AP (JNK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17337-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using JNK Polyclonal antibody (17337-1-AP)

    IF Staining of SH-SY5Y using 17337-1-AP (same clone as 17337-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 17337-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17337-1-PBS in a different storage buffer formulation.

Product Information

17337-1-PBS targets JNK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JNK fusion protein Ag11372
Full Name mitogen-activated protein kinase 9
Calculated Molecular Weight 424 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC032539
Gene Symbol JNK
Gene ID (NCBI) 5601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.