JNK Polyclonal antibody

JNK Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17337-1-AP

Synonyms

MAPK9, JNK2A, JNK2, JNK 55, EC:2.7.11.24

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Validation Data Gallery

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  WB analysis of mouse brain using 17337-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17337-1-AP (JNK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 17337-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 17337-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

17337-1-AP targets JNK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JNK fusion protein Ag11372
• Full Name: mitogen-activated protein kinase 9
• Calculated Molecular Weight: 424 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC032539
• Gene Symbol: JNK
• Gene ID (NCBI): 5601
• RRID: AB_2878388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.

Publications

Species	Application	Title
mouse	WB	Int Immunopharmacol; RG108 attenuates acute kidney injury by inhibiting P38 MAPK/FOS and JNK/JUN pathways; Authors - Fan-Xu Kong
mouse	WB	Adv Sci (Weinh); Ultra-Long-Term Anti-Inflammatory Polyphenol Capsule to Remodel the Microenvironment for Accelerating Osteoarthritis Healing by Single Dosage; Authors - Shaoyin Wei
human	WB	BMC Cancer; Aldo-keto reductase family 1 member C3 mediates radioresistance of esophageal cancer cells through suppressing MAPK and AKT signaling; Authors - Wei Xiong