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JMJD3 Polyclonal antibody, PBS Only

JMJD3 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55354-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JMJD3 Polyclonal antibody (55354-1-AP)

    WB analysis using 55354-1-AP (same clone as 55354-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55354-1-AP (JMJD3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55354-1-PBS in a different storage buffer formulation.

Product Information

55354-1-PBS targets JMJD3 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name jumonji domain containing 3, histone lysine demethylase
Calculated Molecular Weight 177 kDa
Observed Molecular Weight 177 kDa
GenBank Accession NumberNM_001080424
Gene Symbol JMJD3
Gene ID (NCBI) 23135
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

JMJD3, also known as KDM6B, is a 1643 amino acid protein, which belongs to the UTX family. JMJD3 is a Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed:17825402, PubMed:17851529, ). JMJD3 demethylates trimethylated and dimethylated H3 'Lys-27', so it Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529). Histone demethylases are epigenetic actors with a crucial role in cancer by acting as suppressors of tumors or as oncogenes. JMJD3 and UTX (ubiquitously transcribed tetratricopeptide repeat, X chromosome) are transcription activators, being specific H3K27me3 demethylases. JMJD3 is involved in many cellular process such as development, differentiation, senescence and aging by p16, p53 and RB pathways and finally inflammation. Depending on cancer type, JMJD3 expression is increased (prostate and breast cancers, melanoma, gliomas, renal cell carcinoma or decreased (lung, liver, pancreatic, colon and colorectal cancers. This role in carcinogenesis has allowed the development of "epidrugs" to modulate JMJD3 expression (PMID: 29805743 ).