JMJD3 Polyclonal antibody
JMJD3 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 55354-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
Product Information
55354-1-AP targets JMJD3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | jumonji domain containing 3, histone lysine demethylase |
| Calculated Molecular Weight | 177 kDa |
| Observed Molecular Weight | 177 kDa |
| GenBank Accession Number | NM_001080424 |
| Gene Symbol | JMJD3 |
| Gene ID (NCBI) | 23135 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
JMJD3, also known as KDM6B, is a 1643 amino acid protein, which belongs to the UTX family. JMJD3 is a Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed:17825402, PubMed:17851529, ). JMJD3 demethylates trimethylated and dimethylated H3 'Lys-27', so it Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529). Histone demethylases are epigenetic actors with a crucial role in cancer by acting as suppressors of tumors or as oncogenes. JMJD3 and UTX (ubiquitously transcribed tetratricopeptide repeat, X chromosome) are transcription activators, being specific H3K27me3 demethylases. JMJD3 is involved in many cellular process such as development, differentiation, senescence and aging by p16, p53 and RB pathways and finally inflammation. Depending on cancer type, JMJD3 expression is increased (prostate and breast cancers, melanoma, gliomas, renal cell carcinoma or decreased (lung, liver, pancreatic, colon and colorectal cancers. This role in carcinogenesis has allowed the development of "epidrugs" to modulate JMJD3 expression (PMID: 29805743 ).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for JMJD3 antibody 55354-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Inflammation Glycoprotein Nonmetastatic Melanoma Protein B (GPNMB) Ameliorates the Inflammatory Response in Periodontal Disease. | ||
Gene miR-148a-3p regulates adipocyte and osteoblast differentiation by targeting lysine-specific demethylase 6b. | ||
Stem Cells Dev Inhibition of Ezh2 In Vitro and the Decline of Ezh2 in Developing Midbrain Promote Dopaminergic Neurons Differentiation Through Modifying H3K27me3. | ||
Oncol Rep Targeting P16INK4A in uterine serous carcinoma through inhibition of histone demethylation. | ||
Nat Cardiovasc Res α-Ketoglutarate promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction |
