JMJD1C Polyclonal antibody

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  WB analysis using 26637-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26637-1-AP (JMJD1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 26637-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JMJD1C antibody (26637-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

JMJD1C Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 26637-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

TRIP8, TRIP 8, JHDM2C

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Tested Applications

• Positive WB detected in: U-251 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26637-1-AP targets JMJD1C in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JMJD1C fusion protein Ag24348
• Full Name: jumonji domain containing 1C
• Calculated Molecular Weight: 2358 aa, 263 kDa
• Observed Molecular Weight: 285 kDa
• GenBank Accession Number: BC143722
• Gene Symbol: JMJD1C
• Gene ID (NCBI): 221037
• RRID: AB_3669557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15652
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JMJD1C, a member of the lysine demethylase 3 (KDM3) family, is universally required for the survival of several types of acute myeloid leukemia (AML) cells with different genetic mutations.