JAK1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

JAK1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3H7A8

Cat no : 66466-1-PBS

Synonyms

JAK 1, JAK1, JAK1A, JAK1B, Janus kinase 1, JTK3, Tyrosine protein kinase JAK1

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Validation Data Gallery

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  WB analysis using 66466-1-Ig (same clone as 66466-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66466-1-Ig (same clone as 66466-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 66466-1-Ig (same clone as 66466-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66466-1-Ig (same clone as 66466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66466-1-Ig (same clone as 66466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66466-1-Ig (same clone as 66466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66466-1-Ig (same clone as 66466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66466-1-PBS targets JAK1 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: JAK1 fusion protein Ag17940
• Full Name: Janus kinase 1 (a protein tyrosine kinase)
• Calculated Molecular Weight: 1154 aa, 133 kDa
• Observed Molecular Weight: 133 kDa
• GenBank Accession Number: BC132729
• Gene Symbol: JAK1
• Gene ID (NCBI): 3716
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Janus kinase 1(JAK1), is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 is essential for signaling for certain type I and type II cytokines. JAK1 plays a critical role in initiating responses to multiple major cytokine receptor families. Expression of JAK1 in cancer cells enables individual cells to contract, potentially allowing them to escape their tumor and metastasize to other parts of the body.