Islet 1 Recombinant antibody

Islet 1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240833B2

Cat no : 83784-6-RR

Synonyms

ISL1, ISLET1, ISL LIM homeobox 1, Isl 1, Insulin gene enhancer protein ISL-1

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Validation Data Gallery

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  WB analysis using 83784-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83784-6-RR (Islet 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83784-6-RR is Islet 1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Islet 1 fusion protein Ag33541
• Full Name: ISL LIM homeobox 1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: BC031213
• Gene Symbol: Islet 1
• Gene ID (NCBI): 3670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts. Ubiquitinated probably by WWP1 E3 ubiquitin ligase; ubiquitination is followed by protein degradation.