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Islet 1 Recombinant antibody, PBS Only (Detector)

Islet 1 Uni-rAbTM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240833B2

Cat no : 83784-6-PBS

Synonyms

ISL1, ISLET1, ISL LIM homeobox 1, Isl 1, Insulin gene enhancer protein ISL-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Islet 1 Recombinant antibody (83784-6-RR)

    WB analysis using 83784-6-RR (same clone as 83784-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83784-6-RR (Islet 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83784-6-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00771-4

    Sandwich ELISA standard curve of MP00771-4

    Sandwich ELISA standard curve of MP00771-4, Human Islet 1 Recombinant Matched Antibody Pair - PBS only. 83784-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33541. 83784-6-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP00771-4

    Sample test of MP00771-4

    The mean Islet 1 concentration was determined to be 1.05 ng/mL in SH-SY5Y cell extract based on a 3.40 mg/mL extract load.

Product Information

83784-6-PBS targets Islet 1 as part of a matched antibody pair:

MP00771-4: 83784-7-PBS capture and 83784-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Islet 1 fusion protein Ag33541
Full Name ISL LIM homeobox 1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC031213
Gene Symbol Islet 1
Gene ID (NCBI) 3670
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts. Ubiquitinated probably by WWP1 E3 ubiquitin ligase; ubiquitination is followed by protein degradation.