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Integrin Beta 1 Monoclonal antibody, PBS Only

Integrin Beta 1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A7C9

Cat no : 66315-1-PBS

Synonyms

CD29, FNRB, GPIIA, Integrin beta 1, Integrin beta-1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    WB analysis of HT-1080 using 66315-1-Ig (same clone as 66315-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66315-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    WB analysis of HUVEC using 66315-1-Ig (same clone as 66315-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66315-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    WB analysis of HT-1080 using 66315-1-Ig (same clone as 66315-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66315-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66315-1-PBS targets Integrin Beta 1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Integrin Beta 1 fusion protein Ag24476
Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Calculated Molecular Weight 88 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC020057
Gene Symbol ITGB1
Gene ID (NCBI) 3688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.