Integrin Alpha 3 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Integrin Alpha 3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F9G4

Cat no : 66070-1-PBS

Synonyms

CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a

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Validation Data Gallery

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  WB analysis of A549 using 66070-1-Ig (same clone as 66070-1-PBS)

  WB result of Integrin Alpha 3 antibody (66070-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 3 transfected A549 cells. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 66070-1-Ig (same clone as 66070-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (ITGA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66070-1-Ig (same clone as 66070-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66070-1-Ig (same clone as 66070-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66070-1-Ig (same clone as 66070-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66070-1-Ig (same clone as 66070-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66070-1-Ig (same clone as 66070-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66070-1-Ig (same clone as 66070-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66070-1-PBS targets Integrin Alpha 3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig, mouse samples.

• Tested Reactivity: human, pig, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Integrin Alpha 3 fusion protein Ag17293
• Full Name: integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
• Calculated Molecular Weight: 1066 aa, 119 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC150190
• Gene Symbol: Integrin alpha 3
• Gene ID (NCBI): 3675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.