CoraLite® Plus 488-conjugated INS Monoclonal antibody

INS Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

2

CloneNo.

4B6A7

Cat no : CL488-66198

Synonyms

ILPR, INS, IRDN

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Validation Data Gallery

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  IF Staining of mouse pancreas using CL488-66198

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66198 (INS antibody), at dilution of 1:400. The α cells of pancreas was labelled in red with 15954-1-AP (Glucagon antibody).

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Tested Applications

• Positive IF-P detected in: mouse pancreas tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

CL488-66198 targets INS in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: INS fusion protein Ag8630
• Full Name: INS
• Calculated Molecular Weight: 110 aa, 12 kDa
• GenBank Accession Number: BC005255
• Gene Symbol: INS
• Gene ID (NCBI): 3630
• RRID: AB_2883281
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. It is synthesized as a precursor molecule, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus. INS may also exist 36 kDa form corresponding to the hexameric INS form.

Publications

Species	Application	Title
mouse	IF	Endocrinology; Discordant effects of polyamine depletion by DENSpm and DFMO on β cell cytokine stress and diabetes outcomes in mice; Authors - Batoul Hammoud
mouse	IF	bioRxiv; Correlative Imaging for Comprehensive Molecular Mapping of Individual Cell Types in Biological Tissues; Authors - Manxi Yang