Mouse Ins1 Monoclonal antibody, PBS Only

Ins1 Monoclonal Antibody for IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat

Applications

IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H2C11

Cat no : 67668-1-PBS

Synonyms

Ins 1, Ins1

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Validation Data Gallery

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  IHC staining of mouse pancreas using 67668-1-Ig (same clone as 67668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67668-1-Ig (Ins1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67668-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 67668-1-Ig (same clone as 67668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67668-1-Ig (Ins1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67668-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat pancreas using 67668-1-Ig (same clone as 67668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67668-1-Ig (Ins1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67668-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat pancreas using 67668-1-Ig (same clone as 67668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67668-1-Ig (Ins1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67668-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 67668-1-Ig (same clone as 67668-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Ins1 antibody (67668-1-Ig, Clone: 1H2C11 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67668-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67668-1-PBS targets Ins1 in IF, IHC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Ins1 fusion protein Ag29988
• Full Name: insulin I
• Calculated Molecular Weight: 12 kDa
• GenBank Accession Number: NM_008386
• Gene Symbol: Ins1
• Gene ID (NCBI): 16333
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

insulin I is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.