Inhibin beta A-Specific Monoclonal antibody, PBS Only

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  WB analysis of HepG2 using 60352-1-Ig (same clone as 60352-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 60352-1-Ig (same clone as 60352-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (Inhibin beta A-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 60352-1-Ig (same clone as 60352-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (Inhibin beta A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 60352-1-Ig (same clone as 60352-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:650 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 60352-1-Ig (same clone as 60352-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 60352-1-Ig (same clone as 60352-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60352-1-Ig (same clone as 60352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60352-1-Ig (same clone as 60352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 60352-1-Ig (same clone as 60352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 60352-1-Ig (same clone as 60352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60352-1-Ig (same clone as 60352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 60352-1-Ig (same clone as 60352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

Inhibin beta A-Specific Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 60352-1-PBS

Clone No.1F8D8

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

Activin beta A chain, EDF, FRP, INHBA, INHBA-Specific, Inhibin beta A chain, Inhibin beta A-Specific, inhibin, beta A

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Product Information

60352-1-PBS targets Inhibin beta A-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: inhibin, beta A
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: NM_002192
• Gene Symbol: Inhibin beta A
• Gene ID (NCBI): 3624
• RRID: AB_2881461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08476
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Inhibin βA(INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.