Importin Beta Monoclonal antibody, PBS Only

Importin Beta Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1A12B1

Cat no : 67597-1-PBS

Synonyms

KPNB1, Importin subunit beta-1, Importin subunit beta 1, Importin 90, Impnb

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Validation Data Gallery

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  WB analysis using 67597-1-Ig (same clone as 67597-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67597-1-Ig (Importin beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67597-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67597-1-Ig (same clone as 67597-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67597-1-Ig (Importin beta antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67597-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 67597-1-Ig (same clone as 67597-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human Importin Beta (67597-1-Ig, Clone:1A12B1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 67597-1-PBS in a different storage buffer formulation.

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Product Information

67597-1-PBS targets Importin Beta in Indirect ELISA, IHC, WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Importin Beta fusion protein Ag23094
• Full Name: karyopherin (importin) beta 1
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 95-97 kDa
• GenBank Accession Number: BC003572
• Gene Symbol: KPNB1
• Gene ID (NCBI): 3837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)