Importin Beta Monoclonal antibody, PBS Only

Importin Beta Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A12B1

Cat no : 67597-1-PBS

Synonyms

IMB1, Impnb, Importin 90, Importin Beta, Importin subunit beta 1, IPO1, IPOB, karyopherin (importin) beta 1, Karyopherin subunit beta 1, KPNB1, NTF97, Nuclear factor p97, PTAC97

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Validation Data Gallery

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  WB analysis using 67597-1-Ig (same clone as 67597-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67597-1-Ig (Importin beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67597-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67597-1-Ig (same clone as 67597-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67597-1-Ig (Importin beta antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67597-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 67597-1-Ig (same clone as 67597-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human Importin Beta (67597-1-Ig, Clone:1A12B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67597-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67597-1-PBS targets Importin Beta in WB, FC, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Importin Beta fusion protein Ag23094
• Full Name: karyopherin (importin) beta 1
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 95-97 kDa
• GenBank Accession Number: BC003572
• Gene Symbol: KPNB1
• Gene ID (NCBI): 3837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)